precision plus prestained dual colour protein molecular weight marker  (Bio-Rad)

Journal: BMC Molecular Biology

Article Title: The coding/non-coding overlapping architecture of the gene encoding the Drosophila pseudouridine synthase

doi: 10.1186/1471-2199-8-15

Figure Lengend Snippet: Amino acid sequence and expression of the novel MFLα protein subform . (A) Alignment of MFL (top), dyskerin (middle) and MFLα (bottom) amino acid sequences. White letters highlight identical amino acids on a black background, and different but conserved amino acids on a grey background; block letters on a white background indicate different and non-conserved residues. Lines above or below the sequences indicate putative functional domains. NLS: nuclear localisation signal; TruB I, Trub II: regions having homology with members of the bacterial tRNA pseudouridine synthase family; tyr: tyrosine domain containing a putative uracil binding pocket; PUA: a conserved RNA binding domain observed in archaeal, bacterial and eukaryotic RNA modifying proteins. (B) Western blot analysis of extracts from S2 cells. Affinity-purified rabbit polyclonal antibodies recognizing both MFL and MFLα were utilised (see Methods). A major band corresponding to the MFL subform (56 kDa) and a minor band corresponding to the MFLα subform (29 kDa) were detected. Positions of the protein ladder (Precision Plus Prestained Protein Standard Dual Colour; Biorad Laboratories) are shown on the left.

Article Snippet: The protein ladder used in our experiments was the Precision Plus Prestained Protein Standard Dual Colour (Biorad Laboratories).

Techniques: Sequencing, Expressing, Blocking Assay, Functional Assay, Binding Assay, RNA Binding Assay, Western Blot, Affinity Purification